This study evaluated the short-term preservation (up to 24 days) of Pacific white shrimp (Penaeus vannamei) spermatophores at low temperatures (10°C and 24°C) using mineral oil. Sperm viability was measured every two days through fertility, hatching rate, and nauplii malformations after artificial insemination, compared with natural insemination. The results showed a gradual decline in all parameters, but the technique was effective for short-term storage, maintaining good hatchability for up to 18 days at 10°C (>50%) and 8 days at 24°C (>40%). This methodology has been successfully applied for seven years to facilitate controlled crosses and reduce the risk of pathogen contamination.
